IUBio

(none)

Yingchuan Qi y0qi0001 at ATHENA.LOUISVILLE.EDU
Wed Jul 28 23:05:28 EST 1999


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I'm applying monoclonal antibody to mouse tissue sections. When =
anti-mouse-IgG is used as secondary antibody, it comes a very high =
background. What can I do to reduce the binding between the =
anti-mouse-IgG and general IgGs in mouse tissue?

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<DIV><FONT color=3D#000000 face=3DArial size=3D2>I'm applying monoclonal =
antibody to=20
mouse tissue sections. When anti-mouse-IgG is used as secondary =
antibody, it=20
comes a very high background. What can I do to reduce the binding =
between the=20
anti-mouse-IgG and general IgGs in mouse =
tissue?</FONT></DIV></BODY></HTML>

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