There has got to be SOMEthing you can do with those enormous
slugs in that area! Unfortunately, I am not familiar with "nerve
fasicle" assay. Do you mean, simply, nerve bundles? As in
compound action potential? The fact is, most neurotoxins act
synaptically rather than on the axon, so you would need some CNS
(or neuromuscular junction) involvement.
I believe there is a volume in the series "Methods in Neurosciences"
by Academic Press dealing with toxicology that you should investigate.
Who are the likely targets for the toxin? They would be your best bet
for an assay.
Brian MacNevin wrote in message <7mvkbm$3bt$1 at ra.cc.wwu.edu>...
>Greetings, all.
> I am a graduate student at Western Washington University and I'm
trying
>to track down some protocols for assaying neurotoxicity. Prefereably,
I'd
>like to utilize some form of nerve fascicle assay (ease of dissection
and
>electrode connection). But whole-cell electrode assays are not
impossible at
>my school, it seems.
> I have had a bear of a time trying to find literature on the
basics, and
>would appreciate any pointers! The understanding of a local
terrestrial
>gastropod hangs on this research. Did that sound like an important
enough
>reason to try the research? heh heh heh.
>>--
>Brian MacNevin
>Biology Department
>Western Washington University
>Bellingham, WA 98225-9160
>USA
>macnevin at cc.wwu.edu>Tel: (360) 650-7465
>>
