IUBio

Binding Studies & Receptor Numbers

Scott Eliasof eliasof at nwneuro.com
Wed Jan 6 19:57:00 EST 1999


Hello,

I am doing a binding study on an adherent cell line to get receptor
number/cell. Does anyone know whether I need to consider that only half
the cell is exposed to the label (ie, are the receptors on the bottom
half of the cell not functional and therefore my number will be off by a
factor of 2)?

Thanks in advance,

Scott Eliasof
eliasof at nwneuro.com



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