Since we try to perform immunofluorescence with brain
tissue (cryosections) fixed with formaldehyde
autofluorescence is always a problem - doesn't matter
which wavelength we use. I tested the treatment with
sodium borohydride but there is no difference to the
untreated sections.
Does anybody know a method how to reduce the fluorescent
background?
Any hints are welcome,
Ulrike
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Ulrike Gausmann | D-07743 Jena
Institut fuer Anatomie | Germany
Anatomie II | Tel +49 (0)3641 938553
Teichgraben 7 | email ugau at mti-n.uni-jena.de