Instead of using formaldehyde, try *fresh* paraformaldehyde. The
formaldehyde off the shelf tends to autofluoresce, as does old (one day or
more) paraformaldehyde.
On 23 Jan 1998, Ulrike Gausmann wrote:
> Since we try to perform immunofluorescence with brain
> tissue (cryosections) fixed with formaldehyde
> autofluorescence is always a problem - doesn't matter
> which wavelength we use. I tested the treatment with
> sodium borohydride but there is no difference to the
> untreated sections.
> Does anybody know a method how to reduce the fluorescent
> background?
>> Any hints are welcome,
> Ulrike
>> --
> Ulrike Gausmann | D-07743 Jena
> Institut fuer Anatomie | Germany
> Anatomie II | Tel +49 (0)3641 938553
> Teichgraben 7 | email ugau at mti-n.uni-jena.de>>
