It depends on what you are probing for - e-mail me and I can send you a general protocol
that we use in our labs...
gino.miele wrote:
> Hi, I want to do In Situ Hybridisation on mouse brain sections using DIG-labelled
> RNA probes. Problem is my brain tissue has been collected, frozen in liquid N2 and
> then stored at -80 without any pre-treatment. Can anyone give me advice on the best
> method to use for ISHs on brain tissue stored in this way? Thanks in advance.
>> Gino Miele,
>>gino.miele at bbsrc.ac.uk