IUBio

Method for ISH

Dredd dyer at cord.ubc.ca
Sat Apr 11 17:07:00 EST 1998


It depends on what you are probing for - e-mail me and I can send you a general protocol
that we use in our labs...



gino.miele wrote:

> Hi, I want to do In Situ Hybridisation on mouse brain sections using DIG-labelled
> RNA probes.  Problem is my brain tissue has been collected, frozen in liquid N2 and
> then stored at -80 without any pre-treatment.  Can anyone give me advice on the best
> method to use for ISHs on brain tissue stored in this way?  Thanks in advance.
>
> Gino Miele,
>
> gino.miele at bbsrc.ac.uk






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