In article <5c5dli at news-ipg.umds.ac.uk>, Steve Bunting
<s.bunting at UMDS.ac.uk> wrote:
> Dear all,
>> I am currently trying to isolate cells from adult rat striatum, but I am
> obtaining large amounts of debris (myelin etc). Does anyone know how I
> can "clean up" my primary cultures.
>> Any help will be certainly be appreciated.
>> Steve
Hi Steve,
You can try some kind of centrifugation to remove the lipids. I think that
sucrose gradients, Ficoll, Nycodenz or optiprep may help you (these are
available from Pharma).
Good luck,
Andrea.
--
Andrea LeBlanc