Advanced International Immunofluorescence Course
Gargnano '97 (Italy)
The Advanced International Immunofluorescence Course is a
post-doctorate theoretical/practical course, with propedeutical
lectures and practical stages on traditional and confocal
immunofluorescence microscopy and image and ion analysis.
The course will take place in Gargnano (Lake of Garda) from 7
to 10 October 1997. Further information and registration details will
be found at the following Web address
http://imiucca.csi.unimi.it/endomi/ACIF.html
Program:
Morning Tuesday, October 7, 1997
9.00-10.00 Registration of partecipants
10.00-10.45 AN INTRODUCTION TO
IMMUNOCYTOCHEMISTRY AND
IMMUNOFLUORESCENCE (I.Barajon)
10.45-11.15 Coffee Break
11.15-12.00 LIGHT SOURCES AND FLUOROCHROMES
(G. Bottiroli)
12.00-12.45 THE USE OF FLUORESCENCE MICROSCOPE (C.Rumio)
13.00-14.30 Work lunch
Afternoon
14.00-14.45 FLUORESCENCE PHOTOMICROGRAPHY (P.Castano)
14.45-16.00 Practical stages: Photomicrography
16.00-16.30 Coffee Break
16.30-17.30 Practical stages: Photomicrography
Morning Wednesday, October 8, 1997
9.00 - 9.45 IMMUNOCYTOCHEMISTRY AGAINST IMMUNOFLUORESCENCE
(C.J.F.Van Noorden)
9.45-10.30 APPLICATION OF DIGITAL IMAGE ANALYSIS TO FLUORESCENCE
MICROSCOPY (A.Remuzzi)
10.30-11.00 Coffee Break
11.00-11.45 PRINCIPLES OF CONFOCAL MICROSCOPY (G.J.Brakenhoff)
11.45-12.30 GFP AND Ca 2+ ANALYSIS (R. Rizzuto)
12.30-14.00 Work Lunch
Afternoon
14.30-16.00 Practical stages: Photomicrography, Confocal
Microscopy, Ca Analysis, Image Analysis.
16.00-16.30 Coffee Break
16.30-17.30 Practical stages:
Photomicrography, Confocal Microscopy, Ca Analysis,
Image Analysis.
Evening
21.00-23.00 Illustration and discussion of photomicrographies.
Morning Thursday, October 9, 1997
9.00 - 9.45 IMMUNOFLUORESCENCE FOR CELL CULTURES (C.Tacchetti)
9.45-10.30 IMMUNOFLUORESCENCE ON THICK SAMPLES (S.Modina)
10.30-11.00 Coffee Break
11.00-11.45 MULTIPHOTON LASER SCANNING FLUORESCENCE MICROSCOPY
(A.Dixon)
11.45-12.30 MULTIPLE FLUORESCENCE (A. Entwistle)
12.30-14.00 Work Lunch
Afternoon
14.00-17.00 Visit to the Vittoriale of G.D'Annunzio
17.00-19.30 Practical stages: Photomicrography, Confocal
Microscopy, Ca Analysis, Image Analysis.
Evening
20.30 All-together Dinner in a typical Restaurant
Morning Friday, October 10, 1997
9.00 - 9.45 CONFOCAL MICROSCOPY AND FLUORESCENCE(A.Entwistle)
9.45 -11.00 Round Table: THE MULTI-DIMENSIONAL MICROSCOPY
(G.J.Brakenhoff, A.Entwistle, P.Castano, A.Remuzzi, C.J.F.Van
Noorden)
11.00-11.30 Coffee Break
11.30-13.30 Practical stages: Photomicrography, Confocal
Microscopy, Ca Analysis, Image Analysis.
13.30-14.30 Work Lunch
Afternoon
14.30-17.00 Practical stages: Photomicrography, Confocal
Microscopy, Ca Analysis, Image Analysis.
17.30 End of works.
Thank you
Paolo Castano