IUBio

mouse monoclonal to detect mouse proteins

French A. Lewis falewis at mail.webspan.net
Fri Apr 11 14:21:34 EST 1997


	I have a question for those who have done immunohistochemistry on
mouse brain slices.   My question is how to detect the antibody that has
bound.   I have done western blots, and have used either a conjugated
goat-anti mouse or a goat-anti rabbit as a secondary antibody to visualize
where my primary antibody has bound to proteins on the nitrocellulose.   I
am wondering, if using a goat-anti mouse IgG would lead to high background
in IHC due to the presence of endogenous mouse IgG in the brain.

	Thanks in advance for the help,
	French




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