I have a question for those who have done immunohistochemistry on
mouse brain slices. My question is how to detect the antibody that has
bound. I have done western blots, and have used either a conjugated
goat-anti mouse or a goat-anti rabbit as a secondary antibody to visualize
where my primary antibody has bound to proteins on the nitrocellulose. I
am wondering, if using a goat-anti mouse IgG would lead to high background
in IHC due to the presence of endogenous mouse IgG in the brain.
Thanks in advance for the help,
French