IUBio

Ionophoresis- HELP

Samit sc203 at cus.cam.ac.uk
Fri May 17 10:23:16 EST 1996


Hi!
I have been trying to conduct some basic pharmacological experiments `in 
vivo' on the spinal interneurones using multibarrel electrodes made using 
the electrode puller(Narishige)
I am using a 4-barrel electrode with Muscimol.HBr(MHBr) and 
Bucuculline.CH3I(BMI) in 
2 of them and one for stimulating and the 4th for balancing. I wanted to 
know if any one had any ideas about how much retention and ejection 
currents are required for MHBr and BMI ejections. The concentrations were 
10mM and 1mM respectively.
we want to be stimulating with upto 20micro amps of current.
Also how do you get the electrode to be nice and perfect but at the same 
time have a very low impedence to allow a current upto atleast 20 micro amps?
I shall be extremely grateful if you could give some tips in this matter 
as it has held up my work towards the thesis.
thanks.
Samit
sc203 at cus.cam.ac.uk



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