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Neuronal cell lines

User Name spklgut at iop.bpmf.ac.uk
Tue May 14 11:44:42 EST 1996


> Hi, does anyone know of any neuronal cell lines that exists as a suspension 
> culture, a long shot I know. Failing that can anyone give me any details of 
> Human neuronal cell lines (apart from IMR-32 and SNKSHs).
> 
> TIA Ian Mc

hi,

you could try contacting stratagene, they have a human "neuronal" 
cell
line (i think its called hNT) they even have an office in the UK 
phone
0223 420955 (cambridge). don't know how they would grow in 
suspension. 
there is another cell line called NG-108 which also has neuronal
characteristics but in suspension...? hope this helps,

dave

Hi, 

Strategene fo offer human neuronal progenitors, that can be 
established in culture continuously, they grow as monolayer.  Best 
bet for a neuronal cell suspension is by trying the European Tissue 
culture Collection and/or American Tissue Culture Collection both 
available on the net. 

SKNSH cells are phenotypically characterised as Noradrenergic 
cells, there is 3 different cell types within this cell line (i.e. 
neuronal, intermediate and substrate adherent).  They were first 
established by Biedler et al (1973) and have been fully 
characterised by them (Biedler et al, 1989.  J.Cancer Res).  They 
have also isolated a neuronal clone from this parental line called 
5SHSY, but I understand they are very finnickety and slow growing 
in culture.

hope this is useful.

Gusta, Institute of Psychiatry



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