I am using pc12 cells and NGF to induce neurite growth. Currently, a
neurite growth assay involves using cells from a chick embryo and measuring
growth "manually" as it were. However, it may be possible to quantify
growth using competitive PCR and pc12 cells. The problem is that I
haven't found any good genetic indicators for growth. Does anyone have
any references for any genes that have been linked to neurite growth in
pc12 cells? Does anyone know if a genetic "cascade" has been elucidated
for pc12's yet either? Reponse by personal email is appriciated.
Bryan
3btc at Qlink.QueensU.CA