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Acutely Dissociated Neurons

Michelle Lim Michelle.Lim at anu.edu.au
Thu Jan 18 21:21:28 EST 1996

Hi all!!

I would appreciate any helpful hints/suggestions on how to
acutely dissociate hippocampal neurons from 17-21 day old
rats, particularly those of the dentate gyrus region. At the 
moment I'm using papain and I would like to stick to this 
enzyme for now. Neither slice nor whole hippocampal preps
gives sufficient and reliable numbers of cells.


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