On 4 Dec 1996 19:00:02 GMT, William Barnes (lizer at acs.bu.edu) wrote:
: Hello,
: I have been infusing solid drugs into the brain and have been having a
: hard time controlling the rate of diffusion of the drug through brain
: tissue. Does anyone have any comments to offer in this regard? Thanks.
: William Barnes
:lizer at acs.bu.edu
I have a vague recollection of mixing tracers with polyornithine for
extracellular injection. The polyornithine was supposed to increase the
viscosity of the injection bolus and thereby control diffusion of tracer.
I would think something like dextran or polyvinylpyrrolidone might work
the same, but have no idea about the toxicity or osmotic effects.
Maybe someone else on the list has a protocol, or you can search Medline
(I'm thinking mid-1980s) for a published procedure.
--
Jim Hutchins
Assoc Prof Anatomy, Asst Prof Neurology (Research), Univ Mississippi Med Ctr
http://fiona.umsmed.edu/~hutchins/ *** E-mail: hutchins at umsmed.edu
``Battles between academics are so bitter because so little is at stake.''
Robert Maynard Hutchins [no relation]
