You can improve the separation of proteins and other biopolymers in HPLC
systems for ion exchange, hydroxylapatite, and hydrophobic interaction by
monitoring the conductivity of the eluent stream using an inexpensive
flow through conductivity monitor. Proteins normally elute at the same
conductivity for each run and monitoring conductivity of each run can
therefore provide evidence that chromatographic conditions have been
reproduced while giving an accurate picture of the salt gradient for each
run. Can also be used to monitor HPLC column regeneration and monitor
desalting of proteins by gel filtration. Monitoring of eluent pH is also
discussed. For further details contact Lazar Research Labs. Inc. by
emailing service at lazarlab.com or faxing 1-213-931-1434 or viewing the
Lazar web site at http://www.lazarlab.com