IUBio

Neuronal tracing with DiI

Jurgen Hens jhens at ruca.ua.ac.be
Wed Dec 4 18:08:18 EST 1996


Hello,

One of the subjects of my research is to trace neurons with the
fluorescent dye DiI.  I would like to do this by precipitation of DiI
onto glass beads (75µm), which subsequently are applied onto the neurons
of interest.  My question is the following: does anyone have some
suggestions how I can precipitate different layers of DiI crystals onto
one glass bead?

Thanks,

Jurgen.



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