In article <3u75sr$87k at neuro.usc.edu>,
William Sun <william at neuro.usc.edu> wrote:
>-fix briefly (10 min) in paraformaldehyde, wash with PBS
>-block with 20% horse serum, wash
What protein are you staining? If it is a cellular protein, I think you
should permeablize the cell membrane before block. Try 0.1-1% Triton
for 5 min, it may work.
Xin
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long axons and short dendrites ~{VaM;3$!!JwM;6L~}
http://www.acsu.buffalo.edu/~xinguo