We want to build up a patch clamp setup that is going to be used mainly for whole-cell measurements of voltage activated currents in human glial cells and maybe acutely isolated cells. I would be very happy about information regarding isolation procedures for acutely isolated cells in humans. I know there is a paper out for neocortical acutely isolated cells - does anyone have different protocols and is willing to share?
Also, I have heard that doing the isolation procedure and also tissue slicing procedures in ringer containing saccharose instead of Na will enhance slice/cellquality.
Finally, we had some problems with the water robot Narishige micromanipulators I am sure everyone knows who has been working with them. I am at the moment undecided whether to buy the new Narishige oil manipulators. Anyone care to comment on cost effective micromanipulators suitable for patch clamp work?
Rather a lot ofquestions for one post, sorry, however replies much appreciated.