I am attempting to evaluate the effects of manipulating
the intracellular calcium concentration of VMA-6 (vacuolar
membrane ATPase) mutant yeast cells.
Currently, I have used FURA-2AM to measure the [Ca2+]. Now I
am trying to use FURA-2 and DMSO (dimethyl sulfoxide). Where
can I find information on using these chemicals? Is there a
better method of measuring the Calcium concentration?
I appreciate any help you can provide.