Newsgroups: bionet.neuroscience
Subject: Dissociated cell culture of red nucleus from rat
Summary: Request for suggestions on how to culture Red nucleus
Followup-To: bionet.neuroscience
Distribution: world
Organization: Eye Research Inst. of Canada
Keywords: red nucleus, in vitro, rat(s), cell culture
Hello.
I am trying to grow rat motoneurons in culture. The literature
searches I have carried out have yielded only methods for
culturing lower motoneurons while I am interested in upper
motoneurons, specifically from the red nucleus. I wonder if
centrifugation on a metrizamide density gradient coupled with
panning on a monoclonal antibody to the NGF receptor would be
effective. This is the technique used to achieve pure
populations of motoneurons from the ventral spinal cord. I
would greatly appreciate any suggestions. Please send them to
my e-mail address.
Thank You
loukides at camtwh.eric.on.ca
