<ashton at binfc1.jrf.be> writes:
>I have been making hippocampal slices with a McIlwain tissue chopper
>for several years. People tell me that better and more consistent
>slices can be made with a Vibrotome. I see that Microslicers and
>Rotorslicers are also now available for this purpose. Is there
>anybody out there who has used these different techniques and
>would like to advise me which is the best?
I have been doing my honours this past year and have been doing the majority
of the work on brain slices (well, actually, the hippocampal section of a
slice but same thing really). We have a Campden Instruments Vibroslicer and
routinely cut 100 micron thick slices on it quite easily. Mind you I have
done LOTS of slices and I do remember it being frustrating at first but so
is every new technique. If you want to know a little more about it then
email me, okay??
Hope it helps...
Protein Dynamics Unit
Department of Chemistry
Australian National University
bsf651 at cscgpo.anu.edu.au