I'm having no end of trouble trying to stain up some cultures of ventral
mesencephalon/substantia nigra with a rabbit anti-tyrosine hydroxylase
antibody from Chemicon. I was wondering if anyone could recommend
any good antibodies to TH for immunocytochemistry. I have seen papers staining
TH positive cells after fixation in paraformaldehyde fixative with no
glutaraldehyde, and was wondering if perhaps TH is sensitive to glutaraldehyde.
Any advice would be much appreciated.
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Richard Burge | e-mail:
King's College London | R.Burge at bay.cc.kcl.ac.uk
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