Iacob Mathiesen (iacob.mathiesen at basalmed.uio.no) wrote:
: Does anyone know about a dye which is possible to inject (with
: microelectrodes) into living muscle fibres without harming them to much. We
: want to keep the muscles in culture for 1-3 days after the injections are
: made.
: Thank you for answering!
Any of the fluorescent dextrans should work (eg from Molecular Probes) if
you can live with them in your electrodes. We are getting very good
long-term labeling of cultured neurons with PKH26 (Sigma) but have not
tried microinjecting it. Dyes like fluorescein and its derivatives, and
Lucifer Yellow will leak out too fast.