Does anyone have experience doing in situ transcription/T7 amplification
method reported by Eberwine (PNAS vol. 87, 1663 - 1667, 1990 and SCIENCE
vol.240, pp 1661 - 1664, 1988). I have tried this method several times
and have obtained some aRNA in each try. The amount of aRNA I get is
sufficient for it to be used as probe but I have gotten nowhere near the
80 fold molar amplification reported by the authors. Any and all
suggestions of how I can increase my yield are appreciated.