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In situ transcription

sarven sarven at ecc.ubc.ca
Tue Sep 28 22:14:41 EST 1993

Does anyone have experience doing in situ transcription/T7 amplification
method reported by Eberwine (PNAS vol. 87, 1663 - 1667, 1990 and SCIENCE
vol.240, pp 1661 - 1664, 1988).  I have tried this method several times
and have obtained some aRNA in each try.  The amount of aRNA I get is
sufficient for it to be used as probe but I have gotten nowhere near the
80 fold molar amplification reported by the authors.  Any and all
suggestions of how I can increase my yield are appreciated.


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