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Experienced neurobiologist looks for a post doc job in or in the neighbourhood
of Berlin/Germany were preferred. Jobs in clinical research would be also
appreciated.
Description of research experience and list of institutes joined see below.
Closer description and references upon response to
schmidtc at aeolus.ethz.ch
Possible start from March 1994
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Research experience in:
Immunochemical techniques
- ELISA
- Immunoaffinity chromatography
- Immunofluorescence
- Fluorescence activated cell sorting (FACS analysis)
- Production and use of monoclonal and polyclonal antibodies
- Western blotting
Biochemical techniques
- Purification of (glyco)proteins from different tissues
- Purification of (glyco)lipids
- Thin layer chromatography (TLC)
- Enzymatic and chemical cleavage procedures
- Isoelectric focussing
- SDS PAGE and electroelution
- Fast performance liquid chromatography (FPLC)
- RIA (determination of sencond messengers)
Cell culture
- preparation of primary cultures (cerebellar microexplants,
small cerebellar neurons, dorsal root ganglia)
- use of permanent cell lines
- microinjection (Xenopus oocytes, permanent cell lines)
- electroporation
- isolation of neuronal growth cones and synaptosomes
- isolation of cell nuclei
- spectrofluorimetric determination of Ca2+ and pH in
neuronal cell cultures
Data processing
- word processing on IBM and Macintosh
- use of sequence analysis software (e.g. GCG)
- use of IBAS image analysis system
Microscopy
- Transmission and scanning electron micoscopy
- light microscopy
Teaching experience
- training of students in biochemical techniques during
practical courses
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Employment history:
Undergraduate research fellow at the Institute for Cell and Tumor Biology,
German Cancer Research Center (DKFZ), Heidelberg, Germany
Graduate research fellow at the Institute for Neurobiology, University of
Heidelberg
Graduate research fellow at the Institute for Neurobiology, Swiss Federal
Institute of Technology, Zuerich, Switzerland
PhD: end of 1993
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