Can anyone give me some details on the Nissl stain called Toluidine
blue? What I am interested in is why is this particular stain different
from other Nissl stains like cressyl violet? What about toluidine blue
makes it okay for autorads while others are not? Toluidine blue also
stainsl clearly around the nucleus of the cell but the exact boundaries
of the cell are slightly fuzzy? Is this my artifact or a result of the
characteristics of toluidine blue? Thanks in advance!
gregory s fraley