xiaoz at gandalf.rutgers.edu (Xiao Zhang) writes:
>I am trying recording from a cell line of our lab. Although I have some experiences about PC 12 and cultured neuron, I found it is very difficult for me to get signal from this cell line. The problem is that it is very hard to break-in.I tried sucking by mouth or using a syringe, the result is that almost everytimes when I break the membrane, I loss the seal at the same time. Any suggestion on this matter would be very appreciated.
Two things:
Try different glass.
Try using Fl in your internal solution. While this may not be desirable, it
may get you started.
John Zempel
jzempel at morpheus.wustl.edu