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Help on whole cell patch clamp

John Zempel jzempel at morpheus.wustl.edu
Wed Jul 14 20:56:45 EST 1993

xiaoz at gandalf.rutgers.edu (Xiao Zhang) writes:

>I am trying recording from a cell line of our lab. Although I have some experiences about PC 12 and cultured neuron, I found it is very difficult for me to get signal from this cell line. The problem is that it is very hard to break-in.I tried sucking by mouth or using a syringe, the result is that almost everytimes when I break the membrane, I loss the seal at the same time. Any suggestion on this matter would be very appreciated. 

Two things:

Try different glass.

Try using Fl in your internal solution.  While this may not be desirable, it
may get you started.

John Zempel
jzempel at morpheus.wustl.edu

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