I am trying recording from a cell line of our lab. Although I have some experiences about PC 12 and cultured neuron, I found it is very difficult for me to get signal from this cell line. The problem is that it is very hard to break-in.I tried sucking by mouth or using a syringe, the result is that almost everytimes when I break the membrane, I loss the seal at the same time. Any suggestion on this matter would be very appreciated.
Thank you for your help!!
P.S. I usually use 3-5 M electrodes, and the seal resistance is 1-4 G.
Thanks.