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Help on whole cell patch clamp

Xiao Zhang xiaoz at gandalf.rutgers.edu
Wed Jul 14 18:34:48 EST 1993

I am trying recording from a cell line of our lab. Although I have some experiences about PC 12 and cultured neuron, I found it is very difficult for me to get signal from this cell line. The problem is that it is very hard to break-in.I tried sucking by mouth or using a syringe, the result is that almost everytimes when I break the membrane, I loss the seal at the same time. Any suggestion on this matter would be very appreciated. 

Thank you for your help!!

P.S. I usually use 3-5 M electrodes, and the seal resistance is 1-4 G. 


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