In article <531 at news.duke.edu> tbd at neuro.duke.edu (Tristan Davies) writes:
>In article <16NOV199120460769 at wccf.mit.edu> karuzis at wccf.mit.edu (GLENN HOLM) writes:
>-->Anyone out there have suggestions for the ideal embedding medium to surround
>-->small, fragile brain specimens prior to cutting on vibratome or freezing
>-->microtome? I guess the goal is to find something of the hardness and con-
>-->sistency of perfused (4% paraformaldehyde) brain tissue that can be cast as
>-->a block and then sectioned (around 30 microns) to produce easy to handle
>-->free floating sections. Something on the order of liquid brain.
>>>You left out a couple of details:
>1. Is the tissue you're cutting fixed? with what?
>2. How thick do you want your sections?
>3. Are you only using them for immunocytochemistry?
>>OK, three details that I can think of.
>>I would only add that M1 is the best embedding media as far as I am
concerned for rat and rabbit brains prepared non-fixed for 20um
cryosections. It's made by Harris Inc. I believe.
--
****************************************************************************
* James L. Olds Ph.D. Neural Systems Section *
* domain: olds at helix.nih.gov NINDS, NIH, Bethesda, MD. 20892 USA *
****************************************************************************
