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[Microbiology] RV: Carbenicillin

Fernando J. Arteaga Cabello via microbio%40net.bio.net (by fernando.arteagac from incmnsz.mx)
Tue Apr 8 16:50:28 EST 2014


I have not have that problem with my strains but  I would consider the next:

1. The Carbenicillin´s expiration date. It may have lost activity.
2. Be sure both stocks are correctly prepared.
3. I am not sure, but  may be the reporter gene has something to do with it? Is it the traditional Amp(r) gene or something different?

Hope it helps.

Fernando Arteaga.
-----Mensaje original-----
De: microbio-bounces from oat.bio.indiana.edu [mailto:microbio-bounces from oat.bio.indiana.edu] En nombre de Junhua Zhao
Enviado el: martes, 08 de abril de 2014 11:25 a.m.
Para: microbio from net.bio.net
Asunto: [Microbiology] Carbenicillin

Hi all,

We are trying to use Carbenicillin instead of ampicillin for E. coli selection. However, I do see more colonies (20x more) on Carb plates than Amp plates when I plated the same amount of transformed cultures. Does anyone know how to explain it?

Thanks!
JZ
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