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[Microbiology] Re: Isolation of pure bacterial culture from the mixed culture

PiLS via microbio%40net.bio.net (by pils from invalid.ca)
Mon Mar 25 15:57:37 EST 2013

Yogita Labrath <yogitalabrath from gmail.com> à ploppé:
> Please provide your input how can i proceed over the study of isolating the bacteria of my interest from mixed culture. 
> I know following steps: 
> 1. serial dilution and plating on the diluted on the media plate 
> 2. as per the morphological differences in cells separate the cells (I find all the cells similar not much difference) I label all cells 
> 3. Next step is purify the colony by quadrant streaking (please explain over this part how can i proceed over this step?) 
> 4. Screening the purified culture for the suitable biomolecule production. 
> Can any one suggest a more better method than the mentioned, any alternative? 

This is all a bit meaningless if you do not give any specific
information (what you want to isolate, what is your mixed culture, etc)


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