Yogita Labrath <yogitalabrath from gmail.com> à ploppé:
> Please provide your input how can i proceed over the study of isolating the bacteria of my interest from mixed culture.
> I know following steps:
> 1. serial dilution and plating on the diluted on the media plate
> 2. as per the morphological differences in cells separate the cells (I find all the cells similar not much difference) I label all cells
> 3. Next step is purify the colony by quadrant streaking (please explain over this part how can i proceed over this step?)
> 4. Screening the purified culture for the suitable biomolecule production.
>> Can any one suggest a more better method than the mentioned, any alternative?
This is all a bit meaningless if you do not give any specific
information (what you want to isolate, what is your mixed culture, etc)
--
PiLS
