Sudheer,
It could have been some contaminant (ie detergent residue) left on the
glassware. I would just throw it out and make some new medium. If it keeps
happening, then spend time trying to track it down.
Best of luck!
-----------------------------------------------
Eric T. Gillock, Ph.D.
Associate Professor
Department of Biological Sciences
Fort Hays State University
Hays, KS 67601
-----------------------------------------------
From: microbio-request from oat.bio.indiana.edu
To: microbio from magpie.bio.indiana.edu
Date: 09/23/2011 12:08 PM
Subject: Microbio Digest, Vol 75, Issue 3
Sent by: microbio-bounces from oat.bio.indiana.edu
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Today's Topics:
1. Sterilised LB media getting some medicine smell, while I was
growing E.coli culture (sudheer sangeetham)
2. RE: Sterilised LB media getting some medicine smell, while I
was growing E.coli culture (Hiranya Roychowdhury)
----------------------------------------------------------------------
Message: 1
Date: Thu, 22 Sep 2011 18:14:17 +0200
From: sudheer sangeetham <sudheer.pbm07 from gmail.com>
Subject: [Microbiology] Sterilised LB media getting some medicine
smell, while I was growing E.coli culture
To: microbio from magpie.bio.indiana.edu
Message-ID:
<CAAdHTims7EAPaWOyutgXM52H7pXsAKcg+wph=+U_8z
+wWJz02w from mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Guys
I am curious to know one thing that, I sterilised the LB media (which was
little more diluted). I inoculated the cultures with suitable antibiotics
and while I was measuring the OD of bacteria I could smell some medicine
was
there from the media, I really dont understand why it was like that, but
not
like earlier. After the OD was 0.55, i induced with IPTG but unfortunately
i
could nt able to express the protein.
The only mistake I did was LB media was little diluted, except this, I
think
I have done everything right...
With curiosity, I would like to know what could be the problem? If any one
has any idea......
Thanks in advance
--
Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
------------------------------
Message: 2
Date: Thu, 22 Sep 2011 18:57:14 +0000
From: Hiranya Roychowdhury <hroychow from nmsu.edu>
Subject: RE: [Microbiology] Sterilised LB media getting some medicine
smell, while I was growing E.coli culture
To: sudheer sangeetham <sudheer.pbm07 from gmail.com>,
"microbio from magpie.bio.indiana.edu"
<microbio from magpie.bio.indiana.edu>
Message-ID:
<46202CC0C185DC47BC0295CDEDBFFD6F072F3935 from CH1PRD0102MB145.prod.exchangelabs.com>
Content-Type: text/plain; charset="us-ascii"
Medicine smell? Not a very scientific description since olfaction is
somewhat subjective!
Hiranya S. Roychowdhury, Ph.D.
Associate Professor
Health & Public Services
NMSU-Dona Ana Community College
575 527 7725 (office)
________________________________________
From: microbio-bounces from oat.bio.indiana.edu
[microbio-bounces from oat.bio.indiana.edu] on behalf of sudheer sangeetham
[sudheer.pbm07 from gmail.com]
Sent: Thursday, September 22, 2011 10:14 AM
To: microbio from magpie.bio.indiana.edu
Subject: [Microbiology] Sterilised LB media getting some medicine smell,
while I was growing E.coli culture
Hi Guys
I am curious to know one thing that, I sterilised the LB media (which was
little more diluted). I inoculated the cultures with suitable antibiotics
and while I was measuring the OD of bacteria I could smell some medicine
was
there from the media, I really dont understand why it was like that, but
not
like earlier. After the OD was 0.55, i induced with IPTG but unfortunately
i
could nt able to express the protein.
The only mistake I did was LB media was little diluted, except this, I
think
I have done everything right...
With curiosity, I would like to know what could be the problem? If any one
has any idea......
Thanks in advance
--
Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
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