[Microbiology] doubt in reviving bacterial culture from glycerol stock

Kanchanadevi k via microbio%40net.bio.net (by kdevik from gmail.com)
Sat Jun 25 20:33:37 EST 2011

Hi all

I want to revive bacterial culture from glycerol (70%) stock stored in -20
for two years
I tried by inoculating glycerol stock in nutrient agar plate and nutrient
But, No growth has come
so I couldn't revive that culture

can anyone tell me
what is other possible way to revive the culture from glycerol stock?

Thank you so much

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