[Microbiology] Re: Microbio Digest, Vol 73, Issue 6

sajeesh K via microbio%40net.bio.net (by sajeesh.kappachery from gmail.com)
Fri Jul 22 00:21:00 EST 2011


I have few doubts regarding the preparation of YPD solid media for yeast
which contain Polyethylene glycol (PEG) for drought studies.
Doubt 1.
When PEG is added to media before autoclaving it is found to effect the
solidification of media.

When it is added after autoclaving media pH found to alter. What can be done
to solve these issues.

Doubt 2.

While preparing YPD media is it necessary to use buffer like MES buffer as
in case of MS media to maintain the pH (Key point- pH maintenance).

When PEG is added to MS media, MES buffer is also added to media like MS(For
plant) maintain the pH of media. Did you use some buffer in case of YPD
media for yeast culture? If not what measures can be taken to avoid the pH
change while PEG is added to YPD media.

Doubt 3.

What is the pH (Range) of YPD media can be used to grow yeast?  And which is
more ideal?

Please share with me the protocol for the preparation of 10%, 15%, 20% PEG
containing YPD-solid media for yeast.

Thanking you in advance


Sajeesh Kappachery

On Fri, Jul 22, 2011 at 2:05 AM, <microbio-request from oat.bio.indiana.edu>wrote:

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>   1. RE: can glucose and KCN be autoclaved together
>      (Hiranya Roychowdhury)
>   2. MySQL and perl for biologists (Heather Vincent)
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> Message: 1
> Date: Wed, 20 Jul 2011 16:57:37 +0000
> From: Hiranya Roychowdhury <hroychow from nmsu.edu>
> Subject: RE: [Microbiology] can glucose and KCN be autoclaved together
> To: Kanchanadevi k <kdevik from gmail.com>, Microbio
>        <Microbio from magpie.bio.indiana.edu>
> Message-ID:
>        <
> 46202CC0C185DC47BC0295CDEDBFFD6F0712B942 from CH1PRD0102MB145.prod.exchangelabs.com
> >
> Content-Type: text/plain; charset="iso-8859-1"
> Where did you come up with the idea of autoclaving KCN  in the first place?
>  Both the ingredients should be cold sterilized through 02micron sterile
> filter units.  KCN should be kept cold since at room temp it gives out HCN
> fumes. A  stock solution of KCN should be prepared according to the final
> concentration needs, (you can easily make make a 2 to 4% solution (or up to
> approx. 0.6M)  in sterile distilled water cooled to 5-8°C, and perhaps
> further sterilize it as mentioned above.
> Glucose or sucrose char/caramelize in the presence of any salt, but a low
> conc. sugar solution can be autoclaved for up to 15 min, and the
> caramelization doesn't seem to hurt the minimal media much.  But, please do
> not autoclave KCN!  BTW, also do not pipette the medium by mouth.  I had to
> say this.  We have been told to leave mouth-pipetting for the Culicids.
> Hiranya S. Roychowdhury, Ph.D.
> Associate Professor
> Health & Public Services
> NMSU-Dona Ana Community  College
> 575 527 7725 (office)
> ________________________________________
> From: microbio-bounces from oat.bio.indiana.edu [
> microbio-bounces from oat.bio.indiana.edu] on behalf of Kanchanadevi k [
> kdevik from gmail.com]
> Sent: Tuesday, July 19, 2011 10:20 PM
> To: Microbio
> Subject: [Microbiology] can glucose and KCN be autoclaved together
> Hi every one
> Is it possible to autoclave KCN with glucose containing minimal medium
> I read glucose carmelizes while autoclaving by reacting with amino acids.
> So, glucose is always autoclaved separately and added to the required
> medium
> after autoclaving.
> Therefore I have a doubt wheather glucose will have any reaction with KCN
> If
> I autoclave it together with the minimal medium?
> can u please tell if u have any experience regarding this.
> thank you so much in advance
> have great days
> --
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> ------------------------------
> Message: 2
> Date: Wed, 20 Jul 2011 22:53:22 +0100
> From: Heather Vincent <Heather.Vincent from manchester.ac.uk>
> Subject: [Microbiology] MySQL and perl for biologists
> To: microbio from magpie.bio.indiana.edu
> Message-ID: <4E274E52.8060106 from manchester.ac.uk>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> Computational biology, or Biocomputing, is concerned with methods for
> the efficient storage and processing of biological data. A large part of
> the work of computational biologists thus consists of the management and
> analysis of strings of characters. These strings may be nucleic acid or
> protein sequences, or their annotation.
> The University of Manchester's distance learning course in Biocomputing
> introduces unix, perl for data processing, and database design. As we
> begin at an introductory level, no prior programming experience is
> required.  In the second part of the course students will implement a
> database design in MySQL.
> This course, which is just one of our online Masters level courses in
> Digital Biology, will begin again in October.  Our other courses include :
> Bioinformatics for Systems Biology
> Health Informatics
> Computational Systems Biology
> Introduction to software development in Java
> You will find links to all of our online courses here :
> http://octette.cs.man.ac.uk/bioinformatics/modules/index.html  If you
> have questions about any course, please contact the tutor named on the
> course information page.  For general queries about the programme in
> Digital Biology, and to apply, please mail our office for Advanced
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> End of Microbio Digest, Vol 73, Issue 6
> ***************************************

Sajeesh Kappachery
Ph.D. Scholar
Konkuk University, 1 Hwayang-dong
Gwangjin-gu, Seoul, Korea-143 701

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