On Jul 20, 12:20 am, Kanchanadevi k <kde... from gmail.com> wrote:
> Hi every one
> Is it possible to autoclave KCN with glucose containing minimal medium
>> I read glucose carmelizes while autoclaving by reacting with amino acids.
> So, glucose is always autoclaved separately and added to the required medium
> after autoclaving.
>> Therefore I have a doubt wheather glucose will have any reaction with KCN If
> I autoclave it together with the minimal medium?
>> can u please tell if u have any experience regarding this.
>> thank you so much in advance
>> have great days
>> --
I used to make small batches of the base media and autoclave it and
then keep them in the refrigerator until needed. I also had filter
sterilized KCN stored and would add the KCN to the base only when
needed. And yes it was back in the days when we mouth pipetted
everything! I actually felt safer doing it by mouth than trying to
manipulate some of the early pipette helpers!
