[Microbiology] Antibiotic neutralizers in sterility testing

Sheila Vilas Boas Fraga - TASQA via microbio%40net.bio.net (by sfraga from tasqa.com.br)
Thu Nov 25 10:15:35 EST 2010


I'm trying a sterility test of antibiotic (oxytetracycline). This formulation has oxytetracicylne 217mg/mL + formaldehyde 
sodium (2.2mg/mL). I'm using Membrane Filter Method with Fluid (1g/L Peptone + Tween in differents concentrations ex: 7g/L; 1g/L to wash teh membrane), but it isn't comparable with the positive controle. Wil you suggest another neutralizer? 

Sorry my English, I'm learning!! 
Best regards, 

	Laboratórios TASQA	Sheila Vilas Boas Fraga 
Depto. de Biologia 
Laboratórios TASQA 
Tel: +55 19 3874-3710 
Fax: +55 19 2138-8885 
sfraga from tasqa.com.br 	Unidade Paulínia 
Praça 28 de Fevereiro, 55 
13140-000 - Paulínia / SP 

Unidade Ipatinga 
Tel/Fax: +55 31 3823-4433 

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