Hi all,I have a modified RO (reverse osmosis) membrane and I want to check biofilm growth on the membrane surface. For this purpose, I'm using two bacterial strains (E.coli and Pseudomonas aeruginosa) in my system. I want to see and analyze (using confocal microscope) which strain is causing more biofilm on the membrane surface when grown in mixed culture. But, since I'm not microbiologist I don't really know which stains I have to use.
- Do I have to stain each of the bacteria before mixing and growing them in the system (e.g. red for E.coli and green for P.aeruginosa)?
- Are the stains (e.g. SYTO 59) permanent stains?
Thanks a lot for your help!