Katherine, USP has nothing to do with the level of activity characterized by disnfectants.? The concept the USP attempts to address is the carry-over into culture of sufficient preservative that the viable microbes don't grow.? Preservatives, esp. parabens, don't kill as rapidly as disinfecatants - rather they inhibit and the bugs die off.? Success crtieria established by USP specify kill - so one must ensure that the bugs are dead.
It's not clear what the original poster wanted in the "prep test.".? The USP is pretty simple to follow.
-----Original Message-----
From: microbio-request from oat.bio.indiana.edu
To: microbio from magpie.bio.indiana.edu
Sent: Thu, Sep 10, 2009 1:04 pm
Subject: Microbio Digest, Vol 52, Issue 3
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Today's Topics:
1. Re: Microbio Digest, Vol 52, Issue 2 (jorge1907 from aol.com)
2. Re: USP Microbial Limits Prep Test (Christina Benson)
(Kate Hawley)
3. Can any body give some suggetion about growing biofilm on RO
membrane (sajeesh k)
4. hai (subhash subhash)
----------------------------------------------------------------------
Message: 1
Date: Wed, 09 Sep 2009 17:24:35 -0400
From: jorge1907 from aol.com
Subject: [Microbiology] Re: Microbio Digest, Vol 52, Issue 2
To: microbio from oat.bio.indiana.edu
Message-ID: <8CBFFAB1067752F-2DD8-3A49A from webmail-m069.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
What dio you mean "prep" test???
Neutralization is intended to allow detection of viable by not growing bugs.
-----Original Message-----
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Message: 1
Date: Tue, 8 Sep 2009 16:52:
12 -0600
From: "Christina Benson" <cbenson from nutritionallabs.com>
Subject: [Microbiology] USP Microbial Limits Prep Test
To: <microbio from magpie.bio.indiana.edu>
Message-ID: <24031E4AE32244F98F946FA60DED23FC from NLNT>
Content-Type: text/plain; charset="us-ascii"
Hi there,
I was wondering if you would be willing to share the SOP you wrote on how to
perform the prep test? I am having a hard time figuring it out. I also
think it is kind of an oxymoron to be testing antimicrobial stuff.
Antimicrobial is good, right? Why ruin it by neutralizing?
Thanks for your time,
Christina Benson
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------------------------------
Message: 2
Date: Wed, 9 Sep 2009 16:42:53 -0700 (PDT)
From: Kate Hawley <labcat76 from yahoo.com>
Subject: [Microbiology] Re: USP Microbial Limits Prep Test (Christina
Benson)
To: microbio from magpie.bio.indiana.edu
Message-ID: <787873.67102.qm from web52907.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Christina:In testing antimicrobials, neutralization should always be carried out
to define the testing period. ?For example, you are looking at bleach and wonder
how many bacteria are killed in a 15 second contact period. ?You expose the
bacteria to the bleach for 15 seconds, then recover them in neutralizer. ?This
stops the action of the bleach, the test period is defined as 15 seconds. ?Then
plate the solution and wait for the 24 to 48 hours to see how many organisms
survived and calculated your reduction from the inoculum (or zero time) organism
count. ?If you had recovered the organisms in phosphate buffer or water, the
bleach would still be acting on the organisms over the period required to
enumerate the bacteria and your test period now is undefined or at best the
whole 24 to 48 hours it takes to look at the plates. ?Regardless of
the
antimicrobial, whether fast acting like bleach/disinfectants or slower like
silver or triclosan,
neutralization is required for understanding kill times. ?Common neutralizers
include letheen broth, D/E broth, and SCDLP, but you need to determine the
appropriate neutralizer for your antimicrobial.
Katherine Harrell Hawley
Message: 1
Date: Tue, 8 Sep 2009 16:52:12 -0600
From: "Christina Benson" <cbenson from nutritionallabs.com>
Subject: [Microbiology] USP Microbial Limits Prep Test
To: <microbio from magpie.bio.indiana.edu>
Message-ID: <24031E4AE32244F98F946FA60DED23FC from NLNT>
Content-Type: text/plain;??? charset="us-ascii"
Hi there,
I was wondering if you would be willing to share the SOP you wrote on how to
perform the prep test?? I am having a hard time figuring it out.? I also
think it is kind of an oxymoron to be testing antimicrobial stuff.
Antimicrobial is good, right?? Why ruin it by neutralizing?
Thanks for your time,
Christina Benson
------------------------------
Message: 3
Date: Thu, 10 Sep 2009 02:06:58 -0700
From: sajeesh k <sajeesh.kappachery from gmail.com>
Subject: [Microbiology] Can any body give some suggetion about growing
biofilm on RO membrane
To: microbio from magpie.bio.indiana.edu
Message-ID:
<1e0abc6a0909100206o190e83feg839e8cf4e58d013 from mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Respected Reserchers,
I am trying to set a chemostat experimental set up using CDC reacter for
growing Aeromonas hydrophila biofilm on Reverse osmosis membrane.
Can any body give suggetion about the strength of LB media sutable for the
experimnet and the dilution facter.Also how long i shuld run the chemostat
to get a robust biofilm on RO surface.
Thaking you in advance,
Sajeesh
------------------------------
Message: 4
Date: Wed, 9 Sep 2009 23:32:50 -0700
From: subhash subhash <subhash.g.venkat from gmail.com>
Subject: [Microbiology] hai
To: Microbio from magpie.bio.indiana.edu
Message-ID:
<666de2570909092332w5b3f5392rd562894942c21257 from mail.gmail.com>
Content-Type: text/plain; charset=I
SO-8859-1
sirs and madams
here i have one problem with cy3 cy5 fluorescent images they show only light
imaging cntrovercy during epifluorescent microscopic observation.i cont
differentiate the cy5 images from cy3 images. cy5 images are not visualised
under this epifluorescent microscope, so please help to me reduce such
problems during visualisation. if possible explain with diagrams.
with regards
subhash.g.venkat
------------------------------
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