On 2009-11-22 10:37:25 -0500, Shalini selvam <lovey5dovey from hotmail.com> said:
>> Hello there!To anyone concern:
>> I have a question.
>> How long can a media last after being autoclaved and where should we store
> it?can we autoclave it twice?
>> And how do we set up microbial consortia?
>> Thank.
>> Regards,Shalini
>> _________________________________________________________________
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Generally media that has been dispensed into screw capped tubes will
last a lot longer, especially if the caps are screwed tight after the
media has cooled. Petri dish media dries out and needs to be sealed in
a bag or container and usually requires refrigeration.
If you add blood to a media, it will shorten the "expriation date"
significantly. I wouldn't trust blood media past 4 to 6 weeks.
If you make the media in large flasks, you must cool the agar to about
50C and then pour into whaterver vial or plate you intend to use. It is
not recommended to autoclave again since that could carmalize some of
the sugars and may change some chemical properties and pH. You can
remelt media by bringing it above the melting temp of agar which is
around 97C, but don't go over that, just enough to get it all to melt.
There are a few good references for media preparation including the
Difco Manual and the American Society of Micribiology Manual of
Clinical Micribiology. These are the references I've used in my many
years of media making and storing.
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
yjgent from cox.net
