[Microbiology] glycerol stock for bacteria and fungi,

[mohamed eda]

Dear all hope you all fine and doing well
I want to prepare a glycerol stock for bacteria and fungi, and I want to know if there are different techniques  for each, 
the second point is I did not have a -80 degrees freezer only -30 which I have, so it is possible to use it for the storage of the glycerol stock of the isolates and in this case is there is any special precaution in case of using -30 degree freezer.
thanks in advance
                                 Yours
 
Mohamed Eida 
   Agricultural Microbiology Dept. 
   National Research Center, Egypt 
 Hiroshima University   
 Graduate School of Biosphere Science 
Assessment of Environmental Dynamics Dept. 
 Plant Environmental Science Lab.  

phone No. 090-6405-4222  (SoftBank)