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[Microbiology] Re: (no subject)

shiraz14 via microbio%40net.bio.net (by shiraz14 from singnet.com.sg)
Sun Jan 25 07:41:47 EST 2009


On Jan 24, 5:51 am, Bob <bbx107.... from excite.XYZ.com> wrote:
> On Thu, 22 Jan 2009 17:33:00 -0800 (PST), Kate Hawley
>
> <labca... from yahoo.com> wrote:
>
> >I am looking to identify specific bacterial metabolites that cause malodor and then measure those byproducts by GC or other analytical method.  But I am finding it difficult to find those specific by-products.  Most interested in by-products of Staph and E. coli.  We have looked at ammonia and Proteus, measuring with Drager columns, but I want something more sophisticated.  Any ideas?
>
> Take a culture that you find malodorous. Run the headspace gas thru
> GC-MS.
>
> bob
>
>
>
> - Hide quoted text -
>
> - Show quoted text -

Remove the contaminating dominant products first through depletion via
negative column-exchange chromatography. Then do GC-MS as Bob
suggested.

sz


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