hi everyone
i need to digest 16srRNA pcr product with restrication enzyme
can any one tell me commonly used enzyme for 16srRNA pcr product.
actually i have isolated around 50 strains of unknown bacterial sample
and i did 16srRNA amplication with universal primer 1492R, 27F
now i just wanted to see whether these bacterial strain are same or
different for further studies
for i wanted to digest them with restriction enzyme
so can any one tell me which enzyme i can use.
thank u so much
--
K.Kanchana Devi,
Research Scholar,
