[Microbiology] Re: Candida culturing

JEDilworth via microbio%40net.bio.net (by dugrad from nospam.com)
Tue Apr 28 11:10:07 EST 2009

Plate your diluent. You probably have a contaminant.

Also, when setting up from ATCC, it's best to make a couple of passes to 
"wake up" your organism before working with it. Sub a colony from your 
original plate to a new SABS (or similar medium) and let grow. Do this 
again. Put your bug onto a slant to save it (or freeze it using beads, which 
I don't know how to do but someone else may). You can also do it the "old 
way" we used to do - put on a slant and overlay with sterile mineral oil. 
Keeps for about a year at room temperature that way.

Hope this helps.

Judy Dilworth, M.T. (ASCP)

"Christopher M Havens" <CHAVENS from uwyo.edu> wrote in message 
news:mailman.981.1217627990.3533.microbio from net.bio.net...

   I have been having some trouble maintaining cultures of candida which I 
have aquired from ATCC. 

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