Plate your diluent. You probably have a contaminant.
Also, when setting up from ATCC, it's best to make a couple of passes to
"wake up" your organism before working with it. Sub a colony from your
original plate to a new SABS (or similar medium) and let grow. Do this
again. Put your bug onto a slant to save it (or freeze it using beads, which
I don't know how to do but someone else may). You can also do it the "old
way" we used to do - put on a slant and overlay with sterile mineral oil.
Keeps for about a year at room temperature that way.
Hope this helps.
Judy Dilworth, M.T. (ASCP)
"Christopher M Havens" <CHAVENS from uwyo.edu> wrote in message
news:mailman.981.1217627990.3533.microbio from net.bio.net...
I have been having some trouble maintaining cultures of candida which I
have aquired from ATCC.