IUBio

[Microbiology] re:which medium

Harry Thuis via microbio%40net.bio.net (by Harry.Thuis from nvi-vaccin.nl)
Thu Apr 9 10:08:16 EST 2009


We have a problem with the validation of bioburden testing of WFI on R2A 
medium(milliflex system)
We are spiking WFI wih Ps.aeruginosa (in our test  the positive controle 
value was 25 CFU  and the value in WFI was < 10 CFU)
The recovery is les than 50% .
Is this a normal value for this species in WFI?


Harry Thuis
Nederlands Vaccin Instituut
Tel. +31 (0)30-2744334
Fax. +31 (0)30-2744420
E-mail harry.thuis from nvi-vaccin.nl


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