On 2008-05-16 16:04:25 -0400, senaatlaw Atlaw Woldeyohanes
<senaatlaw from yahoo.com> said:
> Dear Sir,
>> I have isolated a Microorganism, Gram-negative Coccobacillus
> Streaked it on MacConkey agar, pink colonies started to grow before
> eight hours.
> Today after 24 hrs, it is Lactose fermenting, good pink colonies,
> and is Oxidase positive too, I thought it would be from
> Enterobactericeae, but it is rare to be Oxidase positive or unless one
> of the rare ?
> I only have the basic id. Material, and I am going to send it for
> identification to another Lab.
> But I am so curious what can it be, I try to find out on my
> Diagnostic Microbiol. Book, nothing matches at this moment I may have
> to see more.
> Mac, Positive and Oxidase positive, if there is some common
> microorganism which matches this or a good reference, would you please
> let me know.
We're going to need a bit more information. Is this a human isolate,
animal or environmental? Did you do basic id media like TSI, Urea,
motility, Indole? Are you sure it is a pure isolate?
Make sure you streak for isolation, select a single colony. Is your
oxidase reagent fresh?
I know of only one human isolate that could (<3%) be lactose pos and
oxidase pos. Also be careful about your Gram's stain morphology.
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
yjgent from cox.net