IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Microbiology] Problem with cultivation on anaerobic solid media

N10 via microbio%40net.bio.net (by limbic_lesion from hotmail.com)
Mon Mar 24 15:06:32 EST 2008


Hi

A silica gel envelope helps

N10

"Mad Twins Dad" <sandro_photo from yahoo.com> wrote in message 
news:mailman.1195.1206380447.2451.microbio from net.bio.net...
>
>
>
> Luzan Tatiana wrote:
>>
>> In my lab we use hungate bottles only for the liquid anaerobic media and
>> bi-
>> layer solid media. We have to do inoculations on solid meida only using
>> Petri dishes in the anaerobic jar supplemented with gas-packs or
>> controlled
>> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag
>> mixture we use is 10% Hydrogen,   15% CO2 and Nitrogen as the rest. While
>> incubating plates inside, the media dries up very quickly and an 
>> enourmous
>> condensation is forming! IT is almost a lake of water there.
>>
>> IS there any product which would absorb an acess condensate?
>> Thanks a lot
>>
>> --
>>
>>   Tanya Luzan
>> Research Assistant
>> Department of Biology
>> University of Louisiana at Lafayette
>> Phone (Office): 482-5056
>>
>> _______________________________________________
>> Microbio mailing list
>> Microbio from net.bio.net
>> http://www.bio.net/biomail/listinfo/microbio
>>
>>
>
> Tanya, I would agree with using sterile selica,
> another option you have - just dry your plate for a while.
> I guess it will be rather enough to leave 1.5% agar plates overnight.
>
> Cheers.
>
> Sasha.
>
> PS I could not reach your email box, sorry.
> -- 
> View this message in context: 
> http://www.nabble.com/Problem-with-cultivation-on-anaerobic-solid-media-tp13756475p16246239.html
> Sent from the Bio.net - Microbio mailing list archive at Nabble.com.
> 




More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net