IUBio

[Microbiology] Problem with cultivation on anaerobic solid media

Mad Twins Dad via microbio%40net.bio.net (by sandro_photo from yahoo.com)
Mon Mar 24 01:54:27 EST 2008




Luzan Tatiana wrote:
> 
> In my lab we use hungate bottles only for the liquid anaerobic media and
> bi-
> layer solid media. We have to do inoculations on solid meida only using 
> Petri dishes in the anaerobic jar supplemented with gas-packs or
> controlled 
> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag 
> mixture we use is 10% Hydrogen,   15% CO2 and Nitrogen as the rest. While 
> incubating plates inside, the media dries up very quickly and an enourmous 
> condensation is forming! IT is almost a lake of water there. 
> 
> IS there any product which would absorb an acess condensate? 
> Thanks a lot 
> 
> --
> 
>   Tanya Luzan 
> Research Assistant 
> Department of Biology
> University of Louisiana at Lafayette
> Phone (Office): 482-5056
> 
> _______________________________________________
> Microbio mailing list
> Microbio from net.bio.net
> http://www.bio.net/biomail/listinfo/microbio
> 
> 

Tanya, I would agree with using sterile selica, 
another option you have - just dry your plate for a while. 
I guess it will be rather enough to leave 1.5% agar plates overnight.

Cheers.

Sasha.

PS I could not reach your email box, sorry.    
-- 
View this message in context: http://www.nabble.com/Problem-with-cultivation-on-anaerobic-solid-media-tp13756475p16246239.html
Sent from the Bio.net - Microbio mailing list archive at Nabble.com.



More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net