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[Microbiology] Problem with cultivation on anaerobic solid media

Mad Twins Dad via microbio%40net.bio.net (by sandro_photo from yahoo.com)
Mon Mar 24 01:54:27 EST 2008




Luzan Tatiana wrote:
> 
> In my lab we use hungate bottles only for the liquid anaerobic media and
> bi-
> layer solid media. We have to do inoculations on solid meida only using 
> Petri dishes in the anaerobic jar supplemented with gas-packs or
> controlled 
> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag 
> mixture we use is 10% Hydrogen,   15% CO2 and Nitrogen as the rest. While 
> incubating plates inside, the media dries up very quickly and an enourmous 
> condensation is forming! IT is almost a lake of water there. 
> 
> IS there any product which would absorb an acess condensate? 
> Thanks a lot 
> 
> --
> 
>   Tanya Luzan 
> Research Assistant 
> Department of Biology
> University of Louisiana at Lafayette
> Phone (Office): 482-5056
> 
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> 

Tanya, I would agree with using sterile selica, 
another option you have - just dry your plate for a while. 
I guess it will be rather enough to leave 1.5% agar plates overnight.

Cheers.

Sasha.

PS I could not reach your email box, sorry.    
-- 
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