[Microbiology] DAPI preparation

[mirzaman zamanzadeh]

Hi,
 
I got a 5-mg DAPI powder from Fluka and I want to prepare a DAPI solution as follows:
 
 





Stock
Vol.
Work
Vol.

µg/mL
mL
µg/mL
mL

40
20
10
80
 
For taking powder, do I need to centrifuge the DAPI powder and then transfer it (5 mg) to a vial for making the stock solution.
 
Thanks in advance,
Mirzaman