I research gram negative bacteria, but I need some information about B. subtilis that I cannot seem to find. I was wondering if anyone could answer some basic questions about this organism and possibly point me to some good review articles about the genetic manipulation and protocols for in in vitro growth.
1) What is the composition of Difco sporulation media? I checked Difco and BD website but couldn't find this product. A google search also yielded no results. I could find no recipe.
2) Why does sporulation medium induce sporulation? Are there less nutrients present?
3) Does B. subtilis grow better at 30 C or 37 C?
Thanks for helping me out.