[Microbiology] Suggestion for carrying out experiments with the genes involved in alkalophily of Bacillus sp.

[shrinivas Dengeti]

I have planned to carry out characterization of genes involved in
alkalophilic nature of thermoalkalophilic Bacillus sp.
Somebody can suggest me some of the methodology involved in PCR
amplification of the genes or preliminary experiments required to
understand molecular basis behind the nature.
D. Shrinivas
Research Scholar